Journal: PLOS Genetics
Article Title: HTL/KAI2 signaling substitutes for light to control plant germination
doi: 10.1371/journal.pgen.1011447
Figure Lengend Snippet: Heatmap representation of mutant germination phenotypes on 20 μM PAC, 20 μM PAC, and 1 μM rac-GR24, 2 μM ABA, or in the absence of light. The germination responses were hierarchically clustered using the complete linkage method with Euclidean Distance. The mutants fall into three groups: det1-1 like (Group 1), abi1-1 like (Group 2), and smax1-2 like (Group 3). The photographs show the bleached seedling phenotype of various mutants grown on 2.5 μM CTL-VI. Bolded cells indicate mutants which showed wild-type sensitivity to CTL-VI. smax1-4 is a novel SMAX1 allele. See for putative mutant list and values.
Article Snippet: A stock solution of rac -GR24, PAC (Cayman Chemical Company, 18864), and ABA (Sigma-Aldrich, A1049-100MG) was diluted in DMSO, 100% ethanol, and 100% methanol, respectively.
Techniques: Mutagenesis